Genetic Assessment of Moroccan Tomato (Solanum lycopersicum L.) Genotypes by RAPD and SSR Markers

  • Rajae Amraoui Laboratory of Biology of Plants and Microorganisms, Faculté des Sciences Oujda – Université Mohammed Premier, BP-717 Oujda, Morocco
  • Dominique Mingeot Laboratory of Wallonia Center of Agronomics Researchs (CRA-W), Département Sciences du Vivant, Bâtiment Jean-Baptiste de La Quintinie, Chaussée de Charleroi, 234 B-5030 Gembloux, Belgium
  • Mohamed Addi Laboratory of Biology of Plants and Microorganisms, Faculté des Sciences Oujda – Université Mohammed Premier, BP-717 Oujda, Morocco
  • Ahmed Elamrani Laboratory of Biology of Plants and Microorganisms, Faculté des Sciences Oujda – Université Mohammed Premier, BP-717 Oujda, Morocco
  • Hana Caid Serghini Laboratory of Biology of Plants and Microorganisms, Faculté des Sciences Oujda – Université Mohammed Premier, BP-717 Oujda, Morocco
  • Aatika Mihamou Laboratory of Biology of Plants and Microorganisms, Faculté des Sciences Oujda – Université Mohammed Premier, BP-717 Oujda, Morocco
  • Malika Abid Laboratory of Biology of Plants and Microorganisms, Faculté des Sciences Oujda – Université Mohammed Premier, BP-717 Oujda, Morocco
Keywords: Moroccan tomato cultivars, RAPD and SSR markers, genetic diversity.

Abstract

For the first time eight local tomato cultivars collected from four different regions of Morocco were assessed with RAPD and SSR methods. Most of RAPD markers give monomorphic banding profiles. Only OPU03 marker showed a total of 4 polymorphic amplicons out of 8 recorded in FIGUIG2 cultivar. The analysis with SSR markers gives more polymorphism. The number of alleles amplified assessed from 2 to 5 alleles among cultivars. The similarity matrix subjected by the unweighted pairgroup arithmetic method (UPGMA) clustering grouped the cultivars in four groups where FIGUIG2 cultivar formed a separate and more distant cluster. In addition this cultivar holds the very high percentage of uniformity (99%) indicating that is an homogeneous traditional cultivar with high purity. This genotype can be conserved and used in breeding programs. More traditional Moroccan cultivars must be collected in order to determine their genetic structure.

Published
2017-06-14
Issue
Section
ARTICLES