Genetic Diversity Analysis of the Natural Populations of Mediterranean Mussels [Mytilus galloprovincialis (Lmk.)] in Agadir Bay: Assessment of the Molecular Polymorphism and Environmental Impact

  • Amal Korrida Equipe de Génétique et Ecologie des Populations Humaines (GEPH), Laboratoire de Biologie Cellulaire et Génétique Moléculaire (LBCGM), Université Ibn Zohr, Faculté des Sciences Agadir, BP 8106 Cité Dakhla, Agadir, Morocco
  • Sami Jamil Jadallah HRH Prince Sultan Bin Abdul Aziz Al Saud International Foundation for Conservation & Development of Wildlife, Department of Genetics, PO Box 116, Agadir, Morocco.
  • Hassan Izaabel Equipe de Génétique et Ecologie des Populations Humaines (GEPH), Laboratoire de Biologie Cellulaire et Génétique Moléculaire (LBCGM), Université Ibn Zohr, Faculté des Sciences Agadir, BP 8106 Cité Dakhla, Agadir, Morocco.
  • Said Benhissoune Laboratoire des Substances Naturelles. Equipe d’Océanographie Biologique, Département de Biologie, Université Ibn Zohr, Faculté des Sciences Agadir, BP 403, Agadir, Morocco.
Keywords: Mytilus galloprovincialis, Cytochrome C Oxidase gene, marine pollution, mussels’ genetic structure

Abstract

Mediterranean mussel (Mytilus galloprovincialis Lmk) has a great environmental and economic importance for Morocco. This work studies the genetic structure and impact of chemical pollution on three different marine populations of Mytilus galloprovincialis that live within Agadir bay. Three collections were made at two clean sites (Cape Ghir and Cape Aglou) and at an impacted site exposed to intense boating and industrial activities (Anza). A 300-bp portion of the mitochondrial DNA coding-region Cytochrome C Oxidase subunit 1 (COI) was studied by polymerase chain reaction (PCR) and DNA sequencing reactions to assess and evaluate amounts of polymorphism in each site. Genetic analysis using COI for 64 individuals showed no significant differentiation between the three subpopulations. AMOVA demonstrated that only 2.83% of variation exists between populations. Besides the genetic evidence presented herein, mussel’s adaptation mechanisms and strategies to marine pollution are also discussed.

Published
2017-05-23
Section
ARTICLES