In Vitro Shoot Regeneration and Development of Microcorms of Moroccan Saffron (Crocus sativus L.)

  • Khalid Lagram Laboratory of Biotechnology and Natural Resources Valorization, Sciences Faculty, Ibn Zohr University, Agadir, Morocco.
  • Mohamed Ben El Caid Laboratory of Biotechnology and Natural Resources Valorization, Sciences Faculty, Ibn Zohr University, Agadir, Morocco.
  • Souad El Aaouam Laboratory of Biotechnology and Natural Resources Valorization, Sciences Faculty, Ibn Zohr University, Agadir, Morocco.
  • Mohamed Lachheb Laboratory of Biotechnology and Natural Resources Valorization, Sciences Faculty, Ibn Zohr University, Agadir, Morocco.
  • Abdelhamid El Mousadik Laboratory of Biotechnology and Natural Resources Valorization, Sciences Faculty, Ibn Zohr University, Agadir, Morocco.
  • Mohammed Amine Serghini Laboratory of Biotechnology and Natural Resources Valorization, Sciences Faculty, Ibn Zohr University, Agadir, Morocco.
Keywords: Crocus sativus, Indirect organogenesis, Microcorm, Moroccan saffron, Plant tissue culture

Abstract

Crocus sativus L. is a male sterile vegetatively propagated plant. Its flower produces stigmas that when dried, constitute the source of a spice commonly known as Saffron. Slow vegetative propagation and diseases limit the production and the development of saffron. “In vitro” culture could be an effective method to overcome these limitations by improving the quantity and the quality of the planting materials. In this work, Crocus sativus L. segments corms of cultivar from the region of Taliouine (Southeast of Morocco) were used for the propagation through indirect organogenesis. To optimize the in vitro growth conditions, we have used the Murashige and Skoog medium (MS medium), supplemented with 2.4-dichlorophenoxyacetic acid (2.4-D) and with 6-benzylaminopurin (BAP) at combination of various concentrations. Our results showed the formation of callus in 85.42% of explants that grow in a culture medium supplemented with 2,4-D combined with BAP, at a concentration of 1mg/l each. In addition, we observed that increasing the concentration of BAP in the culture medium to 1.5mg/l improved the rate of shoots initiation (0.81). In the meantime, we noted that a combination of BAP (8mg/l) and Naphthalene acetic acid (NAA; 2mg/l) has significantly improved the rate of the formation of advanced shoots (6.65). Finally, the shoots that developed were transferred to an induction medium of roots and corms. As a result, we observed that 50% of shoots tested in ½ MS medium supplemented with 2.4-D and of BAP (1 mg/l each) and 5% sucrose, formed corms. Our study provides a first database for in vitro culture of Moroccan saffron cultivars.

Published
2017-06-11
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Section
ARTICLES