The ‘PI 438489B’ by ‘Hamilton’ SNP-Based Genetic Linkage Map of Soybean [Glycine max (L.) Merr.] Identified Quantitative Trait Loci that Underlie Seedling SDS Resistance

  • Kassem My Abdelmajid Plant Genomics & Biotechnology Lab, Department of Biological Sciences, Fayetteville State University, Fayetteville, NC 28301, USA
  • Laura Ramos Department of Plant, Soil, and Agricultural Systems, Southern Illinois University, Carbondale, IL, USA.
  • Leonor Leandro Plant Pathology Department and Agronomy Department, Iowa State University, Ames, IA 50011, USA
  • Gladys Mbofung Plant Pathology Department and Agronomy Department, Iowa State University, Ames, IA 50011, USA
  • David L. Hyten Soybean Genomics and Improvement Lab, 10300 Baltimore Ave, Bldg. 006, Rm. 201, Beltsville, MD 20705, USA
  • Stella K. Kantartzi Department of Plant, Soil, and Agricultural Systems, Southern Illinois University, Carbondale, IL, USA.
  • Robert L Grier IV Plant Genomics & Biotechnology Lab, Department of Biological Sciences, Fayetteville State University, Fayetteville, NC 28301, USA.
  • Victor N. Njiti Department of Agriculture, Alcorn State University, Alcorn State, MS 39096.
  • Silvia Cianzio Plant Pathology Department and Agronomy Department, Iowa State University, Ames, IA 50011, USA
  • Khalid Meksem Department of Plant, Soil, and Agricultural Systems, Southern Illinois University, Carbondale, IL, USA.

Abstract

Soybeans [Glycine max (L.) Merr.] are susceptible to many diseases including fungal diseases such as soybean sudden death syndrome (SDS). Several studies reported SDS resistance quantitative trait loci (QTL) on the soybean genome using different recombinant inbred line (RIL) populations and low density genetic linkage maps. High density exclusively single nucleotide polymorphisms-based (SNP-based) maps were not yet reported in soybean. The objectives of this study were (1) to construct a high density SNP-based genetic linkage map of soybean using the ‘PI438489B’ by ‘Hamilton’ (PIxH, n=50) recombinant inbred line population, and (2) to map QTL for SDS resistance using this high-density reliable genetic SNP-based map. The PI438489B by Hamilton high-density SNP-based genetic map was a high density map composed of 31 LGs, 648 SNPs, and covered 1,524.7 cM with an average of 2.37 cM between two adjacent SNP markers. Fourteen significant QTL were identified for SDS resistance using interval mapping (IM) and composite interval mapping (CIM) with LOD scores that ranged between 2.6 and 5.0. Twelve QTL were identified for foliar disease severity (FDS) and three QTL for root rot severity (RRS) of which one QTL underlain both FDS and RRS. The fourteen QTL were mapped onto ten separate chromosomes of the soybean genome. Seven of the intervals encompassing the QTL had been identified previously (on LGs C1, C2, D1b, G, L, N and O) associated with resistance to SDS but seven were novel (LGs A2 (2), B1, C2, D1a, D1b and O). We constructed the first PI438489B by Hamilton exclusively SNP-Based map and identified fourteen QTL that underlie SDS resistance including both resistances to foliar and root rot symptoms caused by Fusarium virguliforme infection. The QTL discovered here for SDS resistance could be useful to include in breeding programs in developing soybean cultivars resistant to SDS.

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Published
2017-06-15
Section
ARTICLES