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Over-Expression, Purification, and Characterization of Plant LeGST01, the Mammalian GST-Omega Homolog that Interacts with LeTPX1 from a Tomato cDNA Library

Authors

  • Firas Fohely Faculty of Allied Medical Sciences, Palestine Ahliya University (PAU), Jabal Daher, P.O. Box 1041 Bethlehem, Palestine
  • Khaled Sabarna Sabarna 1 Faculty of Allied Medical Sciences, Palestine Ahliya University (PAU), Jabal Daher, P.O. Box 1041 Bethlehem, Palestine; 2 Mediterranean Agronomic Institute of Chania (MAICh), Alsylio Agrokepiou, P.O. Box 85, GR-73100, Chania, Crete, Greece

DOI:

https://doi.org/10.5147/ajb.vi.221

Keywords:

LeTPx1, TPxintB4, over-expression, purification, cDNA library, GST (omega) homologue, hydrogen peroxide, t-butyl hydroperoxide, and cumene hydroperoxide, antioxidant activity.

Abstract

The study focuses on an in vivo GST- omega homologue (pCRT7/TPxII intB4) over-expression, purification and characterization. Experiments purport to characterize the antioxidant activity of the LeTPx1, the interacting glutathione S-transferases BI-GST/GPx, LeGST-T1, T2, T3, T4, T5 and the mammalian inhibitor of apoptosis Bcl-2. Upon specific expression, the proteins exerted, differential protective effects in yeast cells treated with lethal doses of the prooxidants hydrogen peroxide, t-butyl hydroperoxide, and cumene hydroperoxide. The antioxidant activity of LeTPx1 was highest against the cumene hydroperoxide. The overexpressing GST (omega) homologue TPxintB4 (Baier and Dietz, 1999) which share a considerable homology of the mammalian GST-omega1. In conclusion, the work shows that yeast parental strains are extremely sensitive to very low concentrations (0.2mM) of Cumenehydroperoxide. However, after applying the different antioxidants; it appears that the smallest concentrations t to be tolerated. 

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Published

2021-01-03

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How to Cite

Fohely, F., & Sabarna, K. S. (2021). Over-Expression, Purification, and Characterization of Plant LeGST01, the Mammalian GST-Omega Homolog that Interacts with LeTPX1 from a Tomato cDNA Library. Atlas Journal of Biology, 730–740. https://doi.org/10.5147/ajb.vi.221

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ARTICLES