Effect of Carbon, Nitrogen Sources, and Copper Concentration on the Ligninolytic Enzyme Production by Coriolopsis gallica

Abstract

The effect of a wide range of culture conditions on production of laccase and manganese peroxidase by Coriolopsis gallica was studied. Copper, carbon and nitrogen sources, and their concentration had a strong impact on enzyme activities. Several industrially relevant features of the fungus were revealed. Very high enzyme activity was expressed in the cultivation in both synthetic (xylose, glycerol) and lignocellulose (wheat bran)-containing media, and it produced better laccase and MnP at high concentrations (2-3%) of carbohydrates in the absence of any specific inductor. Another feature of this fungus is its capability to secrete laccase during primary metabolism. The fungus proved to be a potent agent of Remazol Brilliant Blue R (RBBR) decolorization.